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csp6i  (New England Biolabs)


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    Structured Review

    New England Biolabs csp6i
    Csp6i, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 136 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/csp6i/CviQI/bio_rxiv__64898__2026__04__12__717890-329-13-14
    Average 95 stars, based on 136 article reviews
    csp6i - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    other:

    Article Title: 3D Chromatin Architecture Re-Wiring at the CDH3/CDH1 Loci Contributes to E-Cadherin to P-Cadherin Expression Switch in Gastric Cancer.
    Article Snippet: Nuclei suspensions were digested with DnpII (New England Biolabs, EVRY cedex, Évry-Courcouronnes, France) as primary and Csp6I (New England Biolabs) as secondary restriction enzymes and re-circularized with T4 DNA Ligase (Thermo Fisher Scientific).

    Article Title: Translocations can drive expression changes of multiple genes in regulons covering entire chromosome arms
    Article Snippet: Cells were then resuspended in 1X NEBuffer r2.1 (NEB; cat. no. B6002S) and digested overnight with 100 U Csp6I (NEB; 10 000 U/mL; #R0639S) at 25°C, 900 RPM, prior to PCI/ethanol precipitation (as Csp6I cannot be heat-inactivated).

    Article Title: Young LINE-1 transposon 5' UTRs marked by elongation factor ELL3 function as enhancers to regulate naïve pluripotency in embryonic stem cells.
    Article Snippet: LINE-1s are the major clade of retrotransposons with autonomous retrotransposition activity.. Despite the potential genotoxicity, LINE-1s are highly activated in early embryos.. Here we show that a subset of young LINE-1s, L1Md_Ts, are marked by the RNA polymerase II elongation factor ELL3, and function as enhancers in mouse embryonic stem cells.

    Article Title: CTCF-mediated cis-regulatory chromatin insulation enforces a central B-cell tolerance checkpoint
    Article Snippet: Cross-links were then reversed by proteinase K treatment, and templates were trimmed with Csp6I (NEB, R0639S) before re-ligation.

    Article Title: 3D Chromatin Architecture Re-Wiring at the CDH3/CDH1 Loci Contributes to E-Cadherin to P-Cadherin Expression Switch in Gastric Cancer
    Article Snippet: Nuclei suspensions were digested with DnpII (New England Biolabs, EVRY cedex, Évry-Courcouronnes, France) as primary and Csp6I (New England Biolabs) as secondary restriction enzymes and re-circularized with T4 DNA Ligase (Thermo Fisher Scientific).

    Purification:

    Article Title: Modeling epigenetic lesions that cause gliomas.
    Article Snippet: Article Modeling epigenetic lesions that cause gliomas

    Article Title: Transitions in chromatin conformation shaped by fatty acids and the circadian clock underlie hepatic transcriptional reorganization in obese mice
    Article Snippet: .. The purified DNA had a second restriction with 200 U of Csp6I (New England BioLabs) in 500 μl reaction volume. ..

    Generated:

    Article Title: Combined loss of CDH1 and downstream regulatory sequences drive early-onset diffuse gastric cancer and increase penetrance of hereditary diffuse gastric cancer.
    Article Snippet: .. 4C-seq libraries were generated, as previously described [36, 37], digested with DnpII (New England Biolabs) and Csp6I (New England Biolabs) and re-circularized with T4 DNA Ligase (Thermo Fisher Scientific). .. 4C-seq libraries were PCR-amplified (supplementary Table 2) and sequenced on Illumina HiSeqX technology.



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